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90 - 100 A

80 – 89 B

70 – 79 C

60 - 69 D

< 60 F

Tentative Grade Allocation:

Homework

~200

Projects

~400

Midterm Exam

~50

Final Exam

~100

Tentative Course Outline

:

1)

Safety and General Lab Protocols

2)

Measuring and Weighing

3)

Pipetting and Accuracy

4)

MSDS and standards for Hazardous and Biohazardous waste.

5)

Review: Flow of Genetic information---Central dogma, Recognition of DNA as

genetic material. Review: DNA replication.

6)

Molecular cloning, methods and tools for studying genes and gene

activity*Introduction to gene manipulation: DNA cloning, restriction enzymes and maps

7)

Molecular cloning, methods and tools for studying genes and gene activity *PCR,

Real time PCR, DNA sequencing. Directed mutagenesis.

8)

Overview: Transcription &posttranscriptional modification in prokaryotic cells

Overview: Transcription &posttranscriptional modification in eukaryotic cells

*Quantifying transcripts

9)

Microaarays. Bioinformatics, Databases and large dataset management.

10) Quality Control/Quality Assurance and GLP/GMP.

Student E-mail Policy:

Dakota College is increasingly dependent upon e-mail as an official form of communication. A

student’s campus assigned e-mail address will be the only one recognized by the campus for

official mailings. The liability for missing or not acting upon important information conveyed via

campus e-mail rests with the student.

Relationship to Campus Theme

:

This course addresses the campus theme by incorporating Biotechnology as applied to natural

resource management, the health sciences, industrial and municipal applications and fish and

wildlife forensics.

Classroom Policies

1) Cell phones, iPods, and related technology are prohibited in the classroom at all times. It is

recommended that you do not bring your cell phone into the classroom or, at the very least, turn

it off.