

a. Describe the function of DNA, RNA, and protein in living cells and the Central
Dogma.
b. Demonstrate how the structure of DNA influences its function, analysis, and
manipulation. Explain and demonstrate the principles involved in DNA analysis
via agarose gel electrophoresis.
Describe previous and current DNA sequencing technologies.
c. Explain the role of enzymes (e.g., restriction enzymes, DNA polymerases, and
nucleases) in the production and manipulation of DNA molecules.
d. Determine and analyze the effect of qualitative and quantitative changes of
specific proteins on cell function.
7) Analyze economic, social, ethical, and legal issues related to
the use of biotechnology.
a. Differentiate between moral, ethical, and legal biotechnology issues.
b. Research ethical issues presented by evolving science, including genetically
modified foods, cloning, bioterrorism, gene therapy, and stem cells.
c. Compare and contrast attitudes about the use of biotechnology regionally,
nationally, and internationally.
d. Evaluate the regulatory policies impacting biotechnology research
‐
e.g., use of
animals in research and applications of recombinant DNA
8) Explore the calibration and maintenance of modern laboratory instrumentation.
9) Study quality assurance/Quality Control and chain of command in lab environments
including GLP/GMP.
Instructor
: Shubham Datta
Office
: NSC 113
Office hours
: M, W, F 11:00-12:50, and by appointment.
Office phone
: 701-228-5463
:
shubham.datta@dakotacollege.eduLecture/Lab Schedule
: Lecture: M,W,F, 10:00-10:50, Lab: Tues, 10:00-11:50
Textbook(s
): None, selected readings and lab protocols.
Course Requirements
: Students are required to complete in class assignments, answer questions
from the text, complete lab assignments where they simulate biotechnology applications in the
real world.
Grading Scale:
Percentage Grade