Spring 2019 Course Syllabi

Course Requirements : Students are required to complete in class assignments, answer questions from the text, complete lab assignments where they simulate biotechnology applications in the real world. Grading Scale: Percentage Grade 90 - 100 A 80 – 89 B 70 – 79 C 60 - 69 D < 60 F Tentative Grade Allocation: Homework ~200 Projects ~400 Midterm Exam ~50 Final Exam ~100 Tentative Course Outline : 1) Safety and General Lab Protocols 2) Measuring and Weighing 3) Pipetting and Accuracy 4) MSDS and standards for Hazardous and Biohazardous waste. 5) Review: Flow of Genetic information---Central dogma, Recognition of DNA as genetic material. Review: DNA replication. 6) Molecular cloning, methods and tools for studying genes and gene activity*Introduction to gene manipulation: DNA cloning, restriction enzymes and maps 7) Molecular cloning, methods and tools for studying genes and gene activity *PCR, Real time PCR, DNA sequencing. Directed mutagenesis. 8) Overview: Transcription &posttranscriptional modification in prokaryotic cells Overview: Transcription &posttranscriptional modification in eukaryotic cells *Quantifying transcripts 9) Microaarays. Bioinformatics, Databases and large dataset management. 10) Quality Control/Quality Assurance and GLP/GMP. Student E-mail Policy: Dakota College is increasingly dependent upon e-mail as an official form of communication. A student’s campus assigned e-mail address will be the only one recognized by the campus for official mailings. The liability for missing or not acting upon important information conveyed via campus e-mail rests with the student. Relationship to Campus Theme :

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