Spring 2019 Course Syllabi

6) Demonstrate how manipulation of nucleic acids through genetic engineering (recombinant DNA and RNA technologies) alters the function of proteins and subsequent cellular processes. These skills are essential skills utilized in the development and analysis of Fish and Wildlife management and conservation plans. Additionally, these are essential skills for technicians working in the health sciences and environmental compliance. a. Describe the function of DNA, RNA, and protein in living cells and the Central Dogma. b. Demonstrate how the structure of DNA influences its function, analysis, and manipulation. Explain and demonstrate the principles involved in DNA analysis via agarose gel electrophoresis. Describe previous and current DNA sequencing technologies. c. Explain the role of enzymes (e.g., restriction enzymes, DNA polymerases, and nucleases) in the production and manipulation of DNA molecules. d. Determine and analyze the effect of qualitative and quantitative changes of specific proteins on cell function. 7) Analyze economic, social, ethical, and legal issues related to the use of biotechnology. a. Differentiate between moral, ethical, and legal biotechnology issues. b. Research ethical issues presented by evolving science, including genetically modified foods, cloning, bioterrorism, gene therapy, and stem cells. c. Compare and contrast attitudes about the use of biotechnology regionally, nationally, and internationally. d. Evaluate the regulatory policies impacting biotechnology research ‐ e.g., use of animals in research and applications of recombinant DNA 8) Explore the calibration and maintenance of modern laboratory instrumentation. 9) Study quality assurance/Quality Control and chain of command in lab environments including GLP/GMP. Instructor : Shubham Datta Office : NSC 114 Office hours : By appointment. Office phone : 701-228-5463 E-mail : shubham.datta@ndus.edu Lecture/Lab Schedule : Lecture: M,W,F, 12:00-12:50, Lab: Tues, 10:00-11:50 Textbook(s ): None, selected readings and lab protocols.